Responsibilities: - Lead， design， and execute experiments aimed at developing next-generation genome editing strategies using CRISPR-based tools and beyond. - Design and produce DNA， RNA， and virus-based reagents for genome editing experiments in human cells. - Apply various genome editing tools at different stages of CAR-T discovery and development. - Develop and execute in-house NGS workflows， including the following: NGS library preparation and quantification. - Lead from the lab to advance novel and differentiated cell therapy products toward clinical milestones， by working collaboratively with internal cross-functional teams and external partners. - Contribute creatively to seed innovation projects through literature review and shape execution tactics. - Recommends changes to project direction based on an understanding of current literature and in-house experimental data. - Accurately capture data in a timely manner， ensuring data integrity and protocol compliance.
Qualifications: - PhD in molecular biology， biochemistry， synthetic biology or a related field. - Extensive experience in the development and implementation of advanced genetic tools - such as genome editing， CRISPR systems， transposons and DNA assembly - with particular emphasis on the challenges associated with applying these tools in mammalian cells. - Proficiency in standard molecular techniques， e.g.， cloning strategies， PCR， library preparation for next generation sequencing， RNA handling， qPCR， etc. - Experience with NGS library prep， characterization and optimization of CRISPR systems or other technologies. - Good coordination and communication skills are required. Highly organized and efficient， able to work independently. - Strong problem-solving skill is preferred. Ability to coordinate parallel tasks across multiple projects， demonstrating prioritization.
