Responsibilities: - Develop and optimize cell therapy platforms， e.g.， CAR-NK， CAR-γδT， to improve the biological function (potency and persistence) of cell therapy products， based on solid understanding of innate cell biology， i.e. immune cell activation， survival， proliferation， and function， and cancer/immune biology in tumor microenvironment. - Lead from the lab to advance novel and differentiated cell therapy products toward clinical milestones， by working collaboratively with internal cross-functional teams and external partners. - Contribute to discovery projects by independently designing and developing well-defined mechanistic experiments and projects. - Design， execute， and interpret functional in vivo， in vitro， and ex vivo assays to understand the mechanism-of-action and play an active role in subsequent scientific discussions to drive decisions. - Independently design sgRNAs， constructs for KO and KI through non-viral (plasmid， self-replicating vectors， EP， LNP) methods. - Develop characterization strategies and implement plans to understand the cellular biology with respect to growth/expansion of cellular subtypes during process/product development in both autologous and allogenic production processes. - Propose novel projects based on internal results， external partners， and published reports and demonstrate leadership through effective communication of study plans/value， and research results.
Qualifications: - Ph.D. in Immunology， Biology， Biological/Chemical Engineering. - Hands on experience with CAR-T， CAR-NK， CAR-γδT， TCR-T cell therapies / immunotherapies， including cell culture， immune cell activation/expansion， viral transduction for various types of immune cells. - Profound skills in in vitro and in vivo immune cell characterization and function assessment， such as， multi-color flow immunophenotype， multiplex cytokine measurement， cytotoxicity assay， and other molecular based assays. - Experience with in vitro technologies to assess lymphocyte functionality including but not limited to multi-parameter flow cytometry， MSD， Luminiex， xCELLigence and IncuCyte-based assays， for phenotypic characterization of T/NK cells， T/NK cell activation， cytotoxicity and proliferation assays is required. - Experience with gene editing in primary cells is preferred (eg. CRISPR-CAS9 editing， lentiviral transduction， retrovirus transduction) - Excellent written and verbal communication skills， detail-oriented organization skills， and team collaboration skills.
