Responsibilitie工作职责 1. responsible for in vitro functional experiments related work, proficient in the culture, freezing and resuscitation of common cell lines and other operations. 2. One of the following work experience is preferred. ① Experience in isolation, culture, and induced differentiation of primary cells such as PBMC or T cells. ② Familiar with flow analysis technology, experience in multi-color flow, experience in peripheral blood lymphocyte analysis and T cell typing. ③ Including but not limited to reporter gene assay, cytokine secretion, cell proliferation, effector target cell co-culture. ④ Familiar with transient and stable transfection techniques. For example, lentiviral invasion, electrotransformation, familiar with monoclonal selection, experience in the construction of stable transient cell lines is preferred. 3. 3. Timely completion of experimental records, with the ability to handle data, regular work reports. 4. Participate in the maintenance and cleaning of laboratory instruments and supplies. 1. 负责体外功能实验相关工作,熟练掌握常见细胞系的培养、冻存和复苏等操作。 2. 有以下工作经验之一者优先。 ① 有原代细胞如PBMC或T细胞的分离、培养、诱导分化等经验。 ② 熟悉流式分析技术,有多色流式经验,有外周血淋巴细胞分析及T细胞分型经验者。 ③ 包括但不限于报告基因实验、细胞因子分泌、细胞增殖、效靶细胞共培养。 ④ 熟悉瞬转、稳转技术。例如慢病毒侵染,电转,熟悉单克隆挑选,有稳转细胞株构建经验优先。 3. 及时完成实验记录,具备数据的处理能力,定期工作汇报。 4. 参与实验室公共仪器和用品的维护及清洁。
Qualifications职位要求 While on-the-job training will be provided, there are some basic requirement: 1. bachelor degree or above in biology and pharmacy related majors; 2. 3 or more years of relevant work experience for Bachelor's degree and 1 or more years for Master's degree; 3. understand cell culture related technology or have related knowledge background, experience in PBMC is preferred; 4. Careful, responsible, stable and passionate about experimental work; 5. strong learning ability and work rationality, good teamwork spirit and service consciousness. 1. 生物及药学相关专业,本科及以上学历; 2. 相关工作经验本科3年及以上,硕士1年及以上; 3. 了解细胞培养相关技术或有相关知识背景,有PBMC经验者优先考虑; 4. 工作细心谨慎,责任心强,稳定且热爱实验工作; 5. 具有较强的学习能力及工作条理性,有良好的团队协作精神及服务意识。